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Table 2 Application of the orfX -LAMP on clinical Staphylococcus strains

From: Rapid and simple detection of methicillin-resistance staphylococcus aureus by orfXloop-mediated isothermal amplification assay

Samples LAMP PCR
MRSA   
Bloodstream 100% (36/36) 94.4% (34/36)
Respiratory tract 98.6% (145147) 91.8% (135/147)
Skin and soft tissue 98.0% (238/243) 92.6% (225/243)
Urinary tract 98.6% (73/74) 91.9% (68/74)
Other 98.5% (65/66) 86.4% (57/66)
Subtotal 98.4% (557/566) 91.7% (519/566)
MSSA 0% (0/25) 0% (0/25)
MRCNS 0% (0/53) 0.0% (2/53)
MSCNS 0% (0/23) 0.0% (1/23)
Subtotal 0% (0/101) 0.0% (3/101)
  1. Samples, Blood culture specimens included blood inoculated into aerobic and/or anaerobic blood culture medium. Tracheal secretion specimens included secretions from the trachea and bronchia and bronchoalveolar lavage fluid specimens. Wound specimens included surgical wound specimens but also specimens from ulcers, fistulae, abscesses, drainage fluids, catheters sites, percutaneous endoscopic gastrostomy insertion sites, and tracheostomas. Urine contained native urine from urinary catheters or bladder puncture and inoculated culture systems. Other specimens consisted of skin swabs specimens (from the nares, axilla, groin, or perianal area), as well as swab specimens of the throat, tonsils, eye, ear, and vagina; body liquids; puncture exudates.