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Table 1 Segregation analysis of the T 1 progeny from reciprocal controlled crosses

From: Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase

 

Number of T1plants

  

Cross

Total

Transgenic (TR)

Transgene-excised

Non-transgenic (NT)

Ratio TR:NT

χ 2

WT × MB18

183

98

23

85

1:0.9

0.92

MB18 × WT

112

61

NA

51

1:0.8

0.89

WT × MB5

42

22

0

20

1:0.9

0.10

MB5 × WT

33

16

NA

17

1:1.1

0.03

WT × MB17

99

67

15

32

3:1.4

2.83

MB17 × WT

85

58

NA

27

3:1.4

2.07

  1. The T1 plants were analyzed by genomic PCR using primers e3&e4, b1&b2, r3&r4, and g1&g2 (see Table 3). MB, switchgrass plants engineered for pollen-specific transgene autoexcision; NA not applicable, WT wild-type plants.