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Table 1 Segregation analysis of the T 1 progeny from reciprocal controlled crosses

From: Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase

  Number of T1plants   
Cross Total Transgenic (TR) Transgene-excised Non-transgenic (NT) Ratio TR:NT χ 2
WT × MB18 183 98 23 85 1:0.9 0.92
MB18 × WT 112 61 NA 51 1:0.8 0.89
WT × MB5 42 22 0 20 1:0.9 0.10
MB5 × WT 33 16 NA 17 1:1.1 0.03
WT × MB17 99 67 15 32 3:1.4 2.83
MB17 × WT 85 58 NA 27 3:1.4 2.07
  1. The T1 plants were analyzed by genomic PCR using primers e3&e4, b1&b2, r3&r4, and g1&g2 (see Table 3). MB, switchgrass plants engineered for pollen-specific transgene autoexcision; NA not applicable, WT wild-type plants.