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Figure 3 | BMC Biotechnology

Figure 3

From: Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase

Figure 3

Schematic illustration of Bxb1 recombinase-mediated transgene autoexcision in pollen. (a) T-DNA of the vector pMBXS824. PCR primers are indicated as r3&r4, b1&b2, g1&g2, e3&e4, and c1&c2 (TableĀ 3), att sites as grey arrows and hybridization probes as grey rectangles. (b) Predicted pollen-specific autoexcision event and T-DNA fragments. POsPS3 drives the bxbNom gene expression during pollen development and POsPS1 drives the gusAeGFP gene expression after autoexcision. Abbreviations: POsPS1 and POsPS3, pollen specific promoters; bxbNom, optimized Bxb1 recombinase gene; P, PstI restriction site; other abbreviations are the same as in FigureĀ 1.

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