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Table 1 Basic transfer operations needed for MMV

From: Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies

Operation (Pipette-dependence modea)

Purpose

Details

PF

1

Input (I-mode)

Liquid charging into MMV wells

Figure 1c

2

Output (O-mode)

a. Transfer

Complete ejection of liquid in wells

Figure 1b and Additional file 6: Figure S5

b. Washing

3

Square transfer (S-mode)

Fractional volume transfer

Additional file 2: Figure S1

4

Z-direction transfer (Z-mode)

Normal transfer of liquid into wells

Figure 1b and Additional file 5: Figure S4

5

X-direction transfer (X-mode)

Division of liquid in all wells

Additional file 7: Figure S6

6

Magnetic beads transfer (M-mode)

a. Beads recovery

Beads recovery

Additional file 8: Figure S7

b. Division of liquid

Division of liquid in all wells

Additional file 9: Figure S8

7

Filtering (F-mode)

Filter-selective transfer

Figure 2a

PD

8

Pipette-dependent transfer (P-mode)

a. Manual

Manual transfer of liquid or beads

Additional file 10: Figure S9

b. Robotic

Robotic transfer of liquid or beads

Additional file 3: Figure S2

  1. aPipette-free (PF) or pipette-dependent (PD).