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Figure 8 | BMC Biotechnology

Figure 8

From: Cytotoxicity of purified listeriolysin O on mouse and human leukocytes and leukaemia cells

Figure 8

Effect of cholesterol and MβCD pre-treatment on Jurkat cells survival. Jurkat cells were preincubated in RPMI 1640 for 1 h at 37°C with cholesterol (26 μM; 10 μg/ml) or MβCD (5 mM) or a mixture of both. Then LLO was added and the sample was incubated for 30 min. LLO cytotoxicity was tested on a Jurkat cell line with PI. Values represent percentage of living cells. LLO concentration dependence was compared. Median values; P25 and P75, n = 4 p < 0.004 between control and cholesterol or MβCD groups.

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