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Figure 4 | BMC Biotechnology

Figure 4

From: Cytotoxicity of purified listeriolysin O on mouse and human leukocytes and leukaemia cells

Figure 4

Activity of purified LLO on Jurkat cell line. LLO concentration dependence, temperature dependence and conditions of storage were compared. Presented values show the percentage of living cells after exposure to LLO. Cytotoxicity of LLO at 22°C and 37°C were compared on preparations without protease inhibitors, and the influence of storage for 25 days (at −70°C) of LLO preparations on cytotoxicity in the presence of protease inhibitors was assessed. FP –freshly prepared LLO preparation; ST- stored LLO preparation. Median values; P25 and P75, n = 3, p < 0.0001 between FP and ST. Differences in cytotoxic activity at 22°C and 37°C were not significant.

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