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Figure 1 | BMC Biotechnology

Figure 1

From: Reduction in C-terminal amidated species of recombinant monoclonal antibodies by genetic modification of CHO cells

Figure 1

Silencing of PAM using siRNA. PAM mRNA expression level after transfections using the siRNAs (Invitrogen, Ambion, as indicated) in the CHO Der2 parental cell line. In comparison to the negative control (−K), there was up to 8-fold reduction in the mRNA expression levels using the si6 Invitrogen siRNA, and up to 5-fold reductions using the si5 and si6 Ambion siRNAs. Overall, better silencing effects were obtained using the Invitrogen siRNAs. The mRNA expression levels were determined using qPCR (calculated per ACTB housekeeping gene), and the data are means ± standard deviations of the two biological replicates.

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BMC Biotechnology

ISSN: 1472-6750

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