Figure 3

Analysis of CD31 expression in Sca-1⁺cells by FCM and identification of Sca-1⁺-enriched cells by IF staining. A. Flow cytometry analyzed the expression of surface markers CD31 in Sca-1⁺ cells. B. All cells were triple-stained for Sca-1 (green), CD31 (red), and DAPI (blue). Bar = 100 μm.