Isolation and enrichment of Sca-1+cells from the mouse heart and in vitro culture. A. Schematic representation of the isolation and enrichment of Sca-1+ cell populations from the mouse heart. B. Efficiency and feasibility of MACS. The proportion of Sca-1+ cells contained in pre-MACS population, Sca-1+-enriched population, and flow-through population analyzed by flow cytometry. The lower two panels show the morphology of cells after MACS under ordinary light microscopy (×100). C. Cells of Sca-1+-enriched populations cultured and passaged in vitro. Morphology of cells under inverted microscopy. Scale bar = 200 μm.