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Table 2 Reactions included in the metabolic network model of CR.pIX cells

From: The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis

Uptake rates
r1, Glc Glc → Glccyt
r2, Pyr Pyr + 0.33 ATPcyt → Pyrcyt
r3, O2 O2 → O2,cyt
r4, Gln Gln + 0.33 ATPcyt → Glncyt
r5, Glu Glu + ATPcyt → Glucyt
r6, Ala Ala + 0.33 ATPcyt → Alacyt
r7, Asp Asp + ATPcyt → Aspcyt
r8, Arg Arg + 0.33 ATPcyt → Argcyt
r9, Asn Asn + 0.33 ATPcyt → Asncyt
r10, Cys Cys + 0.33 ATPcyt → Cyscyt
r11, Gly Gly + 0.33 ATPcyt → Glycyt
r12, His His + 0.33 ATPcyt → Hiscyt
r13, Ile Ile + 0.33 ATPcyt → Ilecyt
r14, Leu Leu + 0.33 ATPcyt → Leucyt
r15, Lys Lys + 0.33 ATPcyt → Lyscyt
r16, Val Val + 0.33 ATPcyt → Valcyt
r17, Met Met + 0.33 ATPcyt → Metcyt
r18, Phe Phe + 0.33 ATPcyt → Phecyt
r19, Pro Pro + 0.33 ATPcyt → Procyt
r20, Ser Ser + 0.33 ATPcyt → Sercyt
r21, Thr Thr + 0.33 ATPcyt → Thrcyt
r22, Trp Trp + 0.33 ATPcyt → Trpcyt
r23, Tyr Tyr + 0.33 ATPcyt → Tyrcyt
Glycolysis
r24, G6P Glccyt + ATPcyt → G6Pcyt
r25, F6P G6Pcyt ↔ F6Pcyt
r26, FBP F6Pcyt + ATPcyt ↔ FBPcyt
r27, DHAP FBPcyt ↔ GAPcyt + DHAPcyt
r28, GAP DHAPcyt ↔ GAPcyt
r29, PG GAPcyt ↔ NADHcyt + ATPcyt + PGcyt
r30, PEP PGcyt↔ PEPcyt
r31, PEP_Pyr PEPcyt → Pyrcyt + ATPcyt
r32, Pyr_Lac Pyrcyt + NADHcyt ↔ Laccyt
r33, PDH Pyrmit + CoAmit → AcCoAmit + CO2,mit + NADHmit
Pentose phosphate pathway
r34, R5P G6Pcyt → R5Pcyt + CO2,cyt + 2 NADPHcyt
TCA cycle
r35, OAA Malcyt ↔ OAAcyt + NADHcyt
r36, Cit AcCoAcyt + OAAcyt → Citcyt + CoAcyt
r37, Fum_Mal Fumcyt ↔ Malcyt
r38, Citmito AcCoAmit + OAAmit → Citmit + CoAmit
r39, OAAmito Malmit ↔ OAAmit + NADHmit
r40, Fum_Malmito Fummit ↔ Malmit
r41, SCoAmito aKGmit + CoAmit → SuccCoAmit + CO2,mit + NADHmit
r42, Fummito SuccCoAmit ↔ Fummit + CoAmit + ATPmit + FADH2,mit
r43, aKGmito Citmit ↔ aKGmit + CO2,mit + NADHmit
Anaplerosis
r44, Ana_PyrI Malcyt → Pyrcyt + CO2,cyt + NADPHcyt
r45, Ana_PyrII Malmit ↔ Pyrmit + CO2,mit + NADPHmit
r46, PC Pyrmit + CO2,mit + ATPmit → OAAmit
Amino acid catabolism
r47, GDH Glumit ↔ aKGmit + Ammmit + NADPHmit
r48, GS Glncyt ↔ Glucyt + Ammcyt + ATPcyt
r49, Alacat Alacyt + aKGcyt ↔ Pyrcyt + Glucyt
r50, Asn_Asp Asncyt ↔ Aspcyt + Ammcyt
r51, Hiscat Hiscyt → Glucyt + 2 Ammcyt + CO2,cyt
r52, Ilecat Ilecyt + aKGcyt + 2 CoAmit + ATPmit → SuccCoAmit + AcCoAmit + Glucyt + NADHmit + FADH2,mit
r53, Leucat Leucyt + aKGcyt + 3 CoAmit + 2 ATPmit → 3 AcCoAmit + Glucyt + NADHmit + FADH2,mit
r54, Lyscat Lyscyt + 2 aKGcyt + 2 CoAmit + NADPHcyt → 2 AcCoAmit + 2 Glucyt + 2 CO2,mit + 2 NADHcyt + 2 NADHmit + FADH2,mit
r55, Metcat Metcyt + Sercyt + CoAmit + 3 ATPcyt + ATPmit → Cyscyt + SuccCoAmit + Ammcyt + NADHmit + CO2,cyt
r56, Phecat Phecyt + O2,cyt + NADPHcyt → Tyrcyt
r57, Procat Procyt ↔ Glucyt + 2 NADHcyt
r58, Thrcat Thrcyt → Pyrmit + Ammmit + CO2,mit + NADHcyt + NADHmit + FADH2,mit
r59, Trpcat Trpcyt + 2 CoAmit + 3 O2,cyt + NADPHcyt → Alacyt + 2 AcCoAmit + Ammcyt + 2 CO2,mit + 2 CO2,cyt + NADHcyt + 2 NADHmit + FADH2,mit
r60, Valcat Valcyt + aKGcyt + CoAmit + ATPmit → SuccCoAmit + Glucyt + CO2,cyt + 3 NADHmit + FADH2,mit
r61, Tyrcat Tyrcyt + aKGcyt + 2 CoAcyt + 2 O2,cyt → Fumcyt + 2 AcCoAcyt + Glucyt + CO2,cyt
r62, Sercat Sercyt ↔ Pyrcyt + Ammcyt
r63, Cyscat Cyscyt + aKGcyt + O2,cyt → Pyrcyt + Glucyt
r64, Aspcat Aspcyt + aKGmit ↔ Glucyt + OAAmit
r65, Argcat Argcyt + aKGcyt → 2 Glucyt + Ureacyt + NADHcyt
MTHF & uric acid synthesis
r66, MTHF_I Sercyt + THFcyt → Glycyt + MTHFcyt
r67, MTHF_II Glycyt + THFcyt → NADHcyt + CO2,cyt + Ammcyt + MTHFcyt
r68, UricAcid Aspcyt + 2 Glncyt + Glycyt + 2 MTHFcyt + 7 ATPcyt + CO2,cyt → UricAcidcyt + Fumcyt + 2 Glucyt + 2 THFcyt
Lipid synthesis
r69, CHLip 18 AcCoAcyt + 18 ATPcyt + 11 O2,cyt + 27 NADPHcyt → CH + 18 CoAcyt + 9 CO2,cyt
r70, PCLip GAPcyt + Sercyt + 27.6 ATPcyt + 17.6 AcCoAcyt + 4 MTHFcyt + 2 NADHcyt + 31.2 NADPHcyt → PC + 17.6 CoAcyt + 4 THFcyt
r71, PELip GAPcyt + Sercyt + 18.6 ATPcyt + 17.6 AcCoAcyt + MTHFcyt + 2 NADHcyt + 31.2 NADPHcyt → PE + 17.6 CoAcyt + THFcyt
r72, PSLip GAPcyt + Sercyt + 18.6 ATPcyt + 17.6 AcCoAcyt + 2 MTHFcyt + 2 NADHcyt + 31.2 NADPHcyt → PS + 17.6 CoAcyt + 2 THFcyt
r73, PGLLip 2 GAPcyt + 17.6 ATPcyt + 17.6 AcCoAcyt + 4 NADHcyt + 31.2 NADPHcyt → PGL + 17.6 CoAcyt
r74, PILip GAPcyt + G6Pcyt + 17.6 ATPcyt + 17.6 AcCoAcyt + 2 NADHcyt + 31.2 NADPHcyt → PI + 17.6 CoAcyt
r75, SMLip 2 Sercyt + 27.8 ATPcyt + 16.8 AcCoAcyt + 3 MTHFcyt + 2 NADHcyt + 29.6 NADPHcyt → SM + 16.8 CoAcyt + 3 THFcyt
r76, DPGLip 3 GAPcyt + 35.2 ATPcyt + 35.2 AcCoAcyt + 6 NADHcyt + 62.4 NADPHcyt → DPG + 35.2 CoAcyt
Release rates
r77, ATPmain ATPcyt → maintenance
r78, Lacout Laccyt → Lac
r79, Alaout Alacyt → Ala
r80, UricAcidout Uric acidcyt → Uric acid
r81, Ureaout Ureacyt → Urea
r82, Ammout Ammcyt → Amm
r83, CO2 out CO2,cyt → CO2
r84, Pyrout Pyrcyt → Pyr
Transport reactions, oxidative phosphorylation
r85, NADHcyt,trans NADHcyt ↔ NADHmit
r86, ATPtrans ATPcyt ↔ ATPmitt
r87, CO2 trans CO2,cyt ↔ CO2,mit
r88, MALtrans Malcyt + Citmit ↔ Malmit + Citcyt
r89, Glutrans Glucyt ↔ Glumit
r90, Pyrtrans Pyrcyt ↔ Pyrmit
r91, aKGtrans aKGcyt ↔ aKGmit
r92, Ammtrans Ammcyt ↔ Ammmit
r93, FADHox O2,cyt + 2 FADH2,mit → 3 ATPmit
r94, NADHmit,trans NADHmit ↔ NADPHmit
r95, NADHox O2,cyt + 2 NADHmit → 5 ATPmit
r96, NADHcyt,trans NADHcyt ↔ NADPHcyt
Synthesis of macromolecules and biomass
r97, μ 0.552 proteins + 0.263 carbohydrates + 0.131 lipids + 0.023 DNA + 0.031 RNA → biomass
Proteins [1 g] = 955.79 Aspcyt + 1344.29 Alacyt + 543.16 Glncyt + + 817.99 Glucyt + 1024.72 Argcyt + 362.10 Asncyt + 19.61 Cyscyt + 913.16 Glycyt + 271.58 Hiscyt + 240.54 Ilecyt + 588.41 Leucyt + 724.21 Lyscyt + 191.97 Valcyt + 114.57 Metcyt + 201.91 Phecyt + 205.97 Procyt + 247.44 Sercyt + 202.07 Thrcyt + 21.17 Trpcyt + 61.87 Tyrcyt + 24046.3 ATPcyt
Lipids [1 g] = 181 CH + 661.4 PC + 250.3 PE + 90.9 PI + 24.9 PS + 12.6 PGL + 81.4 SM + 26.8 DPG
DNA [1 g] = 3009 R5Pcyt + 3912 Aspcyt + 5717 Glncyt + -2106 NADHcyt + 1505 Glycyt + 22569 ATPcyt + 903 NADHmit + -2407 Malcyt + 903 NADPHcyt + -5717 Glucyt + 5417 MTHFcyt + -5417 THFcyt
RNA [1 g] = 3020 R5Pcyt + 3606 Aspcyt + 6316 Glncyt + 293 O2,cyt + -2435 NADHcyt + 1477 Glycyt + 22614 ATPcyt + 586 NADHmit + -2069 Malcyt + -2954 NADPHcyt + -6316 Glucyt + 4431 MTHFcyt + -4431 THFcyt
Carbohydrates [1 g] = 6172.8 G6Pcyt + 21605 ATPcyt