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Table 1 Determined extracellular fluxes for cultivation of CR.pIX cells in comparison with literature data

From: The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis

  CR.pIX STR AGE1.hn Shakera AGE1.hn Tubeb AGE1.hn Tubec HEK293 STRd,f MDCK STRe,f
Time frame 24–97 h 18–90 h 72–169 h 0–74 h 0–72 h 16–80 h
Initial glc conc. [mM] 33 20–25 30 30 15 30
Initial gln conc. [mM] 1.5 5 2 4 1 2
Glc -211.2 ± 10.8g -123.0 -126.9 -429.2 -395.5 -1108.7
Lac 297.6 ± 8.8 263.3 87.6 639.8 439.0 2108.0
Pyr -30.1 ± 0.8 -28.7 -2.9 -76.5 - 0.0
Gln -0.1 ± 0.1 -37.1 -41.2 -95.5 -66.5 -70.9
Amm 12.6 ± 0.9 - - 91.7 48.5 51.8
Glu -1.4 ± 1.1 4.0 0.9 17.0 -1.4 -42.1
Ala 31.6 ± 4.9 17.5 8.0 20.6 11.8 -49.4
Arg -9.9 ± 2.8 -8.2 -4.4 -12.4 -15.3 -11.7
Asn -17.1 ± 8.5 -2.9 -2.0 -7.3 -4.6 -1.9
Asp -17.8 ± 3.2 -14.1 -8.9 -16.2 -0.2 -2.0
Cys 0.0 0.2 -1.1 -0.2 - -2.4
Gly -4.8 ± 4.8 2.6 -0.8 6.8 17.0 2.3
His -2.3 ± 0.4 -1.7 -0.7 -3.2 -3.5 -2.8
Ile -6.2 ± 4.4 -8.1 -1.4 -14.7 -21.8 -12.3
Leu -7.9 ± 3.2 -11.5 -3.2 -23.8 -24.7 -23.5
Lys -5.5 ± 0.3 -6.9 -3.5 -16.6 -12.6 -22.9
Met -4.1 ± 2.0 -3.1 -1.2 -6.6 -4.9 -6.4
Phe -2.5 ± 1.0 -3.3 -1.5 -6.3 -5.4 -8.9
Pro -0.1 ± 0.1 7.1 13.1 13.4 0.8 6.7
Ser -8.3 ± 8.3 -12.2 -5.7 -33.4 -17.7 -16.8
Thr -3.4 ± 1.9 -4.9 -2.0 -8.0 -9.5 -14.9
Trp -1.5 ± 0.2 -0.9 -0.4 -0.8 - -5.2
Tyr -1.8 ± 1.8 -2.8 -1.4 -5.5 -4.6 -5.0
Val -4.3 ± 2.8 -8.4 -1.8 -14.2 -19.1 -17.0
Uric acid 0.0 - - - - -
  1. All rates are given in μmol/gDW/h.
  2. afrom [9]; bfrom [36]; cfrom [10]; dfrom [7]; efrom [13, 14].
  3. fthese fluxes were re-calculated to [μmol/gDW/h] with the given fluxes and cell dry weights.
  4. gstandard deviation from Monte-Carlo sampling using validated standard deviations of the methods as boundaries.