Table 1 Determined extracellular fluxes for cultivation of CR.pIX cells in comparison with literature data
From: The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis
CR.pIX STR | AGE1.hn Shakera | AGE1.hn Tubeb | AGE1.hn Tubec | HEK293 STRd,f | MDCK STRe,f | |
|---|---|---|---|---|---|---|
Time frame | 24–97 h | 18–90 h | 72–169 h | 0–74 h | 0–72 h | 16–80 h |
Initial glc conc. [mM] | 33 | 20–25 | 30 | 30 | 15 | 30 |
Initial gln conc. [mM] | 1.5 | 5 | 2 | 4 | 1 | 2 |
Glc | -211.2 ± 10.8g | -123.0 | -126.9 | -429.2 | -395.5 | -1108.7 |
Lac | 297.6 ± 8.8 | 263.3 | 87.6 | 639.8 | 439.0 | 2108.0 |
Pyr | -30.1 ± 0.8 | -28.7 | -2.9 | -76.5 | - | 0.0 |
Gln | -0.1 ± 0.1 | -37.1 | -41.2 | -95.5 | -66.5 | -70.9 |
Amm | 12.6 ± 0.9 | - | - | 91.7 | 48.5 | 51.8 |
Glu | -1.4 ± 1.1 | 4.0 | 0.9 | 17.0 | -1.4 | -42.1 |
Ala | 31.6 ± 4.9 | 17.5 | 8.0 | 20.6 | 11.8 | -49.4 |
Arg | -9.9 ± 2.8 | -8.2 | -4.4 | -12.4 | -15.3 | -11.7 |
Asn | -17.1 ± 8.5 | -2.9 | -2.0 | -7.3 | -4.6 | -1.9 |
Asp | -17.8 ± 3.2 | -14.1 | -8.9 | -16.2 | -0.2 | -2.0 |
Cys | 0.0 | 0.2 | -1.1 | -0.2 | - | -2.4 |
Gly | -4.8 ± 4.8 | 2.6 | -0.8 | 6.8 | 17.0 | 2.3 |
His | -2.3 ± 0.4 | -1.7 | -0.7 | -3.2 | -3.5 | -2.8 |
Ile | -6.2 ± 4.4 | -8.1 | -1.4 | -14.7 | -21.8 | -12.3 |
Leu | -7.9 ± 3.2 | -11.5 | -3.2 | -23.8 | -24.7 | -23.5 |
Lys | -5.5 ± 0.3 | -6.9 | -3.5 | -16.6 | -12.6 | -22.9 |
Met | -4.1 ± 2.0 | -3.1 | -1.2 | -6.6 | -4.9 | -6.4 |
Phe | -2.5 ± 1.0 | -3.3 | -1.5 | -6.3 | -5.4 | -8.9 |
Pro | -0.1 ± 0.1 | 7.1 | 13.1 | 13.4 | 0.8 | 6.7 |
Ser | -8.3 ± 8.3 | -12.2 | -5.7 | -33.4 | -17.7 | -16.8 |
Thr | -3.4 ± 1.9 | -4.9 | -2.0 | -8.0 | -9.5 | -14.9 |
Trp | -1.5 ± 0.2 | -0.9 | -0.4 | -0.8 | - | -5.2 |
Tyr | -1.8 ± 1.8 | -2.8 | -1.4 | -5.5 | -4.6 | -5.0 |
Val | -4.3 ± 2.8 | -8.4 | -1.8 | -14.2 | -19.1 | -17.0 |
Uric acid | 0.0 | - | - | - | - | - |