Putative transcription factor binding sites in the 5′-flanking region of the monkey 20α-HSD gene. A) Sequences approximately 2.5 kb upstream from the translation start codon of the monkey 20α-HSD gene. Putative transcription factor binding sites were identified using the TFsearch software. The region includes GR, Ap-1, Oct-1, Sp-1, CRE-BP1 and others. The TATA (-52 → -55) box and two putative CCAAT (-59 → -63; -105 → -109) box sites are indicated in bold. B) The translation start codon is indicated as +1. The region between -350 and -53 has a cluster of transcription factor binding sites that are believed to be important for the basal expression of the monkey 20α-HSD gene.