Bactericidal activities of GM flax seedcake extract on pathogenic bacteria clinical strains

BMC Biotechnology

Table 3 The total phenolic content, antioxidant potential of dried seedcake extract preparation from wild type and modified flax plants and effect on reference bacterial strains

	WT seedcakes dried extract	W92 seedcakes dried extract
	50 mgDW/ml preparation	50 mgDW/ml preparation	30 mgDW/ml preparation	10 mg/ml preparation
Total phenolics content [mg/ml]	24.26 ± 0.03	31.59 ± 0.05	18.45 ± 0.03	6.03 ± 0.06
IC-50 [μl]*	27.5 ± 0.04	10.56 ± 0.03	18.15 ± 0.03	26.36 ± 0.04
K. pneumoniae ATCC 700603**	64% ± 3.03	100% ± 0.00	98% ± 0.01	72% ± 0.93
S. aureus ATCC 29213**	52% ± 2.01	100% ± 0.01	93% ± 1.03	60% ± 0.63
P. aeruginosa ATCC 27853**	46% ± 3.50	100% ± 0.00	100% ± 0.02	85% ± 1.05
E. coli ATCC 25922**	75% ± 1.89	100% ± 0.23	87% ± 1.83	48% ± 1.82

The antimicrobial activity of seedcake preparations were tested on reference bacterial strains. The low concentration of seedcake preparation from control plants (10 mg/ml and 30 mg/ml) do not show any antibacterial properties (data not shown).
*The antioxidant potential (IC50) was defined as the amount (μl) of preparation that inhibits luminol chemiluminescence by 50%. **The presented results are the inhibition rate compared to the untreated bacteria (% of growth inhibition) after 6 h treatment. The results are the mean values ± SD (n = 5).

ISSN: 1472-6750