Table 2 Dectection and efficiency of CRISPR/Cas9-mediated homologous recombination at Ramp2 locus at blastocyst stage
From: A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
Experiment no. | Site injected1 | Cas9 mRNA: R2 gRNA: R2HR donor DNA (ng/μL) | No. zygotes injected | No. Blastocysts/no. zygotes cultures (%) | No. EGFP-expressing blastocysts/no. blastocysts tested (%) |
|---|---|---|---|---|---|
1 | pn | 100:50:10 | 22 | 15/22 (68) | 1/15 (7) |
2 | cyto | 100:50:10 | 20 | 8/16 (50) | 2/8 (25) |
3 | pn/cyto | 200:50:10 | 25 | 7/20 (35) | 4/7 (57)2 |