Table 1 Detection and efficiency of CRISPR/Cas9-mediated indel mutation at Ramp2 locus at blastocyst stage
From: A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
Experiment no. | Site injected1 | Cas9 mRNA: R2 gRNA (ng/μL) | No. zygotes injected | No. blastocysts/no. zygotes cultures (%) | No. mutant blastocysts/no. blastocysts tested (%)2 |
|---|---|---|---|---|---|
1 | cyto | 100:50 | 25 | 19/21 (90) | 6/21 (32) |
2 | pn/cyto | 100:50 | 15 | 8/10 (80) | 2/8 (25) |
3 | pn/cyto | 200:50 | 24 | 8/17 (47) | 8/8 (100)3 |