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Table 2 Cross reactions of α-85 B antibodies with other 85 complex antigens

From: Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B

sequence comparison (corresponding sequences to α-85 B epitopes)

Clone

85 A

85 B

85 C*

85 D

Expected cross reaction

MFU50-A10

KEDPAWQRNDPL

SSDPAWERNDPT

SSDPAWKRNDPM

SDPA

85 A, 85 C, 85 D

MFU50-C10

SPALYL

SPAVYL

PHAVYL

PHAVYL

85 A, 85 C, 85 D

MFU50-D4

AFSRPGLPV

AFSRPGLPV

AFSRPGLPV

-

85 A, 85 C

MFU50-D7

AFSRPGLPVEYL

AFSRPGLPVEYL

AFSRPGLPVEYL

-

85 A, 85 C

MFU50-E2

KEDPAWQRN

SSDPAWERN

SSDPAWKRN

SDPA

85 A, 85 C, 85 D

Reaction with 85 complex antigens in ELISA

Clone

85 A

85 B

85 C*

85 D

Determined cross reaction

MFU50-A10

Strong

Strong

-

Strong

85 A, 85 D

MFU50-C10

None

Strong

-

None

None

MFU50-D4

Weak

Strong

-

None

Weak 85 A

MFU50-D7

None

Weak

-

None

None

MFU50-E2

None

Weak

-

None

None

  1. *Purified antigen 85 C was not available for immunological assays, nevertheless it is listed for completeness of the illustration.