Figure 7From: Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85BDetection of boiled 85 B by α-85 B direct ELISA. 100 ng of antigens in PBS (boiled for 20 min at 100°C or directly taken from storage at 2 – 8°C) were coated to the wells. After blocking bound antigen was detected using MFU50-A10/-C10/-D4-scFv-Fc-HRP at ~0.05 μg/mL and development with TMB. Negative control BSA A450 = 0.02.Back to article page