Figure 4From: Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85BReaction of α-85 B scFv-Fc with Mtb/BCG cell extracts and culture filtrates determined by indirect ELISA. 100 ng/100 μL of the different samples were directly coated to the well, reaction was determined with a scFv-Fc concentration of 2.5 μg/mL. Detection of bound scFv-Fc with goat α-human IgG(Fc)-HRP. Development with TMB.Back to article page