Figure 11

Detection of 85B by immunoblot. A) A-85 B immunoblot and B) Tape Station analysis of concentrated Mtb culture filtrates (22 d and 76 d at 37°C). A) 100 ng of recombinant 85 B, 25 μL of concentrated Mtb culture filtrates (after 22 or 76 days at 37°C in Sauton’s medium) and 8 μL of marker (M) were separated on reducing 12% SDS-PAGE and electro blotted to a PVDF membrane. After blocking, blots were incubated with scFv-Fc-HRP, development with TMB. B) Samples were directly analysed by reducing gel analysis via Tape Station.