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Figure 1 | BMC Biotechnology

Figure 1

From: Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B

Figure 1

Analysis of antigen binding. A) Antibody titration and B) antigen titration in ELISA with α-85 B scFv-Fc. A) Dilution series of scFv-Fc were used for detection of directly coated antigen (85 B) or BSA (negative control). Detection of bound scFv-Fc with goat α-human IgG(Fc)-HRP and development with TMB. B) Different dilutions of antigen (85 B) were directly coated to the wells. Antigen detection with a scFv-Fc concentration of half maximal saturation. Detection of bound scFv-Fc with goat α-human IgG(Fc)-HRP, development with TMB. Negative control BSA A450 = 0.05.

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