Skip to main content

Table 1 Plasmids and strains used in this study

From: Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli

Plasmid or strain Relevant characteristics Source
Plasmids   
pET-22b(+) f1 ori, T7 promoter, AmpR Novagen
pET-28a(+) f1 ori, T7 promoter, KanR Novagen
pET-opTAL pET-28a(+) carrying codon-optimized Saccharothrix espanaensis TAL Kang et al.[31]
pET-CCL pET-28a(+) carrying CCL from Streptomyces coelicolor Choi et al.[18]
pET-KSTS pET-28a(+) carrying codon-optimized Arachis hypogaea STS Choi et al.[18]
pET22-sbCOM1 pET-22b(+) carrying codon-optimized Sorghum bicolor sbOMT1 This study
pET22-sbCOM3 pET-22b(+) carrying codon-optimized Sorghum bicolor sbOMT3 This study
pET-opTLS pET-28a(+) carrying opTAL, CCL, and KSTS This study
pET-opTLO1S pET-28a(+) carrying opTAL, CCL, KSTS, and sbCOM1 This study
pET-opTLO3S pET-28a(+) carrying opTAL, CCL, KSTS, and sbCOM3 This study
pET-opTLO13S pET-28a(+) carrying opTAL, CCL, KSTS, sbCOM1, and sbCOM3 This study
Stranis   
E. coli DH5a cloning host Invitrogen
E. coli C41(DE3) derivative strain of E. coli BL21(DE3) Miroux B & Walker JE [30]