Table 1 Plasmids and strains used in this study
Plasmid or strain | Relevant characteristics | Source |
|---|---|---|
Plasmids | Â | Â |
pET-22b(+) | f1 ori, T7 promoter, AmpR | Novagen |
pET-28a(+) | f1 ori, T7 promoter, KanR | Novagen |
pET-opTAL | pET-28a(+) carrying codon-optimized Saccharothrix espanaensis TAL | Kang et al.[31] |
pET-CCL | pET-28a(+) carrying CCL from Streptomyces coelicolor | Choi et al.[18] |
pET-KSTS | pET-28a(+) carrying codon-optimized Arachis hypogaea STS | Choi et al.[18] |
pET22-sbCOM1 | pET-22b(+) carrying codon-optimized Sorghum bicolor sbOMT1 | This study |
pET22-sbCOM3 | pET-22b(+) carrying codon-optimized Sorghum bicolor sbOMT3 | This study |
pET-opTLS | pET-28a(+) carrying opTAL, CCL, and KSTS | This study |
pET-opTLO1S | pET-28a(+) carrying opTAL, CCL, KSTS, and sbCOM1 | This study |
pET-opTLO3S | pET-28a(+) carrying opTAL, CCL, KSTS, and sbCOM3 | This study |
pET-opTLO13S | pET-28a(+) carrying opTAL, CCL, KSTS, sbCOM1, and sbCOM3 | This study |
Stranis | Â | Â |
E. coli DH5a | cloning host | Invitrogen |
E. coli C41(DE3) | derivative strain of E. coli BL21(DE3) | Miroux B & Walker JE [30] |