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Figure 6 | BMC Biotechnology

Figure 6

From: Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli

Figure 6

Accumulation of 3,4’-dimethoxy-5-hydroxystilbene and 3,5,4’-trimethoxystilbene in the culture of E. coli harboring pET-opTLO13S. A. HPLC profile of the time-course cultivation (14, 20, 36, and 64 hours) of E. coli harboring pET-opTLO13S. The cell was cultured in modified M9 medium at 26°C for (a), 14 hours; (b), 20 hours; (c), 36 hours; (d), 64 hours. p, 4-coumaric acid; peak 1, resveratrol; peak 3, 3,5-dihydroxy-4’-methoxystilbene; peak 5, 3,4’-dimethoxy-5-hydroxystilbene; peak 6, 3,5,4’-trimethoxystilbene. B. Production pattern of one, bis, and tri-methylated resveratrol in the each culture time. , resveratrol; ■, 3,5-dihydroxy-4’-methoxystilbene; ♦, 3,4’-dimethoxy-5-hydroxystilbene; ♦. 3,5,4’-trimethoxystilbene. The concentrations were determined through the use of the corresponding chemical standards. All experiments were performed in triplicates.

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