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Figure 3 | BMC Biotechnology

Figure 3

From: Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli

Figure 3

HPLC profile (A) and selected mass ion chromatogram (B) of bioconversion experiments. A; HPLC profile of the (a) standard resveratrol, (b) pinostilbene, and (c) pterostilbene; (d) resveratrol supplemented E. coli harboring pET22-sbCOM1; (e) resveratrol supplemented E. coli harboring pET22-sbCOM3. The absorbance was monitored at 300 nm. Peak 1, resveratrol; peak 2, pinostilbene; peak 3, 3,5-dihydroxy-4’-methoxystilbene; peak 4, pterostilbene. B; Selected mass ion chromatogram of (a) 3,5-dihydroxy-4’-methoxystilbene (m/z 243.05 [M + H]+) produced by E. coli harboring pET22-sbCOM1; (b) pinostilbene (m/z 243.08 [M + H]+) and (c) pterostilbene (m/z 257.13 [M + H]+) produced by E. coli harboring pET22-sbCOM3. The minor peak of 15.9 min in the HPLC profile (d) was not seen in the mass ion peaks of pterostilbene.

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