Screening and genetic characterization of thermo-tolerant Synechocystis sp. PCC6803 strains created by adaptive evolution

Table 2 Matrix of true-positive mutations (marked with X) identified in the various thermo tolerant strains

Position	Mutation type	Gene	WT	Mutant	Strains
					C5K	A1	A6	H2	H12
96437	AA change	pnp	gAg	gGg	X*
98129	AA change	pnp	aTg	aCg	X*	X			X*
195523	AA change	slr1098	gGc	gTc	X*
494155	AA change	PilJ	Cgg	Tgg			X	X*	X*
1103495	AA change	pyk2	ttC	ttA				X*
1103586	AA change	pyk2	gGa	gAa	X	X			X*
1133812	Intergenic	Intergenic	G	T		X
1436521	Frameshift	cya1	-	GCAA		X
2260225	Intergenic	Intergenic	-	A	X	X			X*
2466798	AA change	clpC	gTg	gCg					X*
2466808	AA change	clpC	Cgt	Tgt	X*	X
2467723	AA change	clpC	Gac	Tac	X*
2468031	AA change	clpC	gTt	gCt	X*
2579829	AA change	sll0064	Ttg	Atg	X*				X*
2717197	Frameshift	pyrR	-	TAATTAACTCCAC	X	X
3371838	AA change	SigF	Cgt	Tgt			X	X*	X

Mutations marked with *indicate mixed reads in Sanger (& NGS), where the wt variant can also still be detected.

ISSN: 1472-6750