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Table 1 Most successful combination of parameters for P. morum transformation using a PDS-1000/He biolistic device

From: Stable nuclear transformation of Pandorina morum

Parameter Parameter specification
Material of microprojectiles gold
Size of microprojectiles 0.6 μm in diameter
Selectable marker plasmid pPmr3
Coating of microprojectiles plasmid-DNA/microcarrier/CaCl2/spermidine/ ethanol -precipitation
Target cells immobilized on cellulose acetate membrane filter; almost free of liquid
Burst pressure of rupture disk 1350 psi
Rupture disk-macrocarrier distance 7 mm
Macrocarrier-stopping screen distance 8 mm
Stopping screen-target cell distance 6 cm
Chamber evacuation 27 inch Hg
Cultivation after particle bombardment in liquid medium