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Figure 4 | BMC Biotechnology

Figure 4

From: Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese encephalitis virus for a subunit vaccine candidate

Figure 4

Production of the JEV-VLP antigen by BJ-ME cells. (A) Confluent BJ-ME cells were incubated with the maintaining medium for 10 days. The amount of JEV-VLP antigen produced each day (24 h) was determined by ELISA. (B) The culture medium of confluent BJ-ME cells was refreshed every 4 to 6 days continuously, and the amount of JEV-VLP antigen in culture supernatants harvested multiple times was determined by ELISA. (C) Effect of long-term passage on production of JEV-VLP antigen by BJ-ME cells. BJ-ME cells were passaged every 3 days in a 75 cm2 flask, and the amount of antigen produced by BJ-ME cells from the different passages was analyzed using ELISA.

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BMC Biotechnology

ISSN: 1472-6750

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