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Table 5 Combinations of GenSolve, Qiagen and NucleoSpin protocols for DNA extraction from DBS: GQ versus NN-XS methods

From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling

      65°C, 65 min  
Sample code DNA (ng/μl) DNA (ng) 260/280 260/230 260/280 260/230 Protocol
NCS 4a 17.5 734 1.98 0.96 1.84 0.98 GQ
NCS 4b 17.3 833 1.72 0.84 1.65 0.78 NN-XS
NCS 5a 17.6 741 1.82 1.05 1.81 1.07 GQ
NCS 5b 12.8 614 1.61 1.02 1.73 1.07 NN-XS
NCS 6a 8.2 328 2.02 0.81 1.79 0.76 GQ
NCS 6b 15.7 756 1.70 0.89 1.70 0.86 NN-XS
NCS 4-10a 22.2 956 1.95 1.21 2.02 1.19 GQ
NCS 4-10b 13.5 660 1.84 1.10 1.85 1.08 NN-XS
NCS 4-11a 19.1 822 1.60 0.85 1.71 0.88 GQ
NCS 4-11b 12.6 618 1.60 1.00 1.58 1.11 NN-XS
NCS 4-12a 10.4 447 1.54 0.74 1.76 0.74 GQ
NCS 4-12b 13.1 657 1.55 0.76 1.70 0.74 NN-XS
  1. Two punches, each having 9 mm diameter, were analyzed per DBS. Punches labeled “a” were tested with GQ while their matched pairs, labeled “b”, were tested with NN-XS. Average eluate volumes by GQ and NN-XS were 42 μl and 49 μl, respectively. Data represent averages of 2–4 readings per sample.