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Table 3 Combinations of GenSolve, Qiagen and NucleoSpin protocols for DNA extraction from DBS: GQ versus GN-XS and Gn-XS methods

From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling

       65°C, 65 min   
  Sample code DNA (ng/μl) DNA (ng) 260/280 260/230 260/280 260/230 Protocol
  NCS 28a 20.3 873 1.84 0.95 1.82 0.97 GQ
NCS 28b 37 1813 1.87 0.11 1.78 0.13 GN-XS
NCS 27a 19.4 834 1.85 0.85 1.80 0.90 GQ
NCS 27b 27.9 1339 2.19 0.61 2.26 0.61 GN-XS
NCS 26a 19.7 847 1.83 0.97 1.74 0.93 GQ
NCS 26b 23.7 1161 1.66 0.79 1.77 0.80 GN-XS
NCS 25a 16.3 701 1.9 1.07 1.79 1.07 GQ
NCS 25b 21.7 1042 1.83 0.34 1.96 0.28 GN-XS
NCS 24a 11.3 486 1.82 0.78 1.79 0.82 GQ
NCS 24b 21.6 1037 1.53 0.08 1.48 0.07 GN-XS
NCS 23a 11.9 512 1.18 0.49 1.27 0.54 GQ
NCS 23b 11.4 547 1.44 0.50 1.52 0.42 GN-XS
Precipitation buffer in GN-XS changed to ethanol, leading to protocol Gn-XS NCS 19a 30.1 1174 1.53 0.71 1.57 0.81 GQ
NCS 19b 15.1 725 1.49 0.65 1.50 0.70 Gn-XS
NCS 18a 12.2 488 1.81 0.62 1.78 0.64 GQ
NCS 18b 20.4 979 1.30 0.64 1.44 0.72 Gn-XS
NCS 17a 59 2360 1.80 1.37 1.91 1.56 GQ
NCS 17b 42.6 2130 1.65 0.53 1.75 0.57 Gn-XS
Washing volume and frequency in GN-XS increased NCS 16a 16.8 823 1.71 0.76 1.80 0.78 GQ
NCS 16b 13.1 707 1.43 0.21 1.55 0.22 GN-XS
NCS 15a 27.1 1382 1.76 1.04 1.79 0.97 GQ
NCS 15b 12.6 668 7.83 0.39 Error * 0.45 GN-XS
NCS 14a 30.3 1545 1.77 1.01 1.83 1.07 GQ
NCS 14b 22.8 1208 2.37 0.60 2.60 0.61 GN-XS
  1. Two punches, each having 9 mm diameter, were analyzed per DBS. Punches labeled “a” were tested with GQ while their matched pairs, labeled “b”, were tested with GN-XS. When the DNA precipitation buffer in GN-XS was changed to ethanol, the resultant protocol was termed Gn-XS. For NCS 16b, 15a and 14b, the washing volume was increased from 100 to 500 μl, and washing was performed twice instead of once. Average eluate volumes by GQ and GN-XS/Gn-XS were 42 μl and 48 μl, respectively. Data represent averages of 2–4 readings per sample. * The error represents values out of range.