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Table 2 Combinations of GenSolve, Qiagen and NucleoSpin protocols for DNA extraction from DBS: GQ versus GN and Gn methods

From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling

       65°C, 65 min   
  Sample code DNA (ng/μl) DNA (ng) 260/280 260/230 260/280 260/230 Protocol
  NCS 31a 5.77 242 1.70 0.42 1.55 0.41 GQ
NCS 31b 8.87 426 1.81 0.95 1.54 0.71 GN
NCS 30a 12.8 538 1.88 0.79 1.76 0.76 GQ
NCS 30b 24.4 1171 1.84 1.22 1.90 1.51 GN
NCS 29a 20.7 828 1.85 1.13 1.76 1.00 GQ
NCS 29b 23.1 1063 1.93 1.38 1.85 1.37 GN
Precipitation buffer in GN changed to ethanol, leading to protocol Gn NCS 22a 9.85 394 1.81 0.50 1.79 0.48 GQ
NCS 22b 8.00 368 1.90 0.83 1.79 0.75 Gn
NCS 21a 24.3 972 1.83 0.84 1.83 0.90 GQ
NCS 21b 27.7 1274 1.64 0.54 1.83 0.61 Gn
NCS 20a 15.0 600 1.78 0.56 1.77 0.60 GQ
NCS 20b 14.4 662 1.70 0.76 1.74 0.89 Gn
  1. Two punches, each having 9 mm diameter, were analyzed per DBS. Punches labeled “a” were tested with GQ while their matched pairs, labeled “b”, were tested with GN. When the DNA precipitation buffer in GN was changed to ethanol, the resultant protocol was termed Gn. Average eluate volumes by GQ and GN/Gn were 42 μl and 47 μl, respectively. Data represent averages of 2–4 readings per sample.