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Table 1 Combinations of Gensolve and Qiagen protocols for DNA extraction from DBS: GQ, QQ and Qq methods

From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling

       65°C, 65 min   
  Sample code DNA (ng/μl) DNA (ng) 260/280 260/230 260/280 260/230 Protocol
  NCS 11a 21.9 944 2.03 1.04 1.89 1.02 GQ
NCS 11b 3.4 147 1.55 0.19 1.35 0.29 QQ
NCS 12a 19.1 802 1.91 1.19 1.90 1.18 GQ
NCS 12b 6.1 257 1.58 0.36 1.73 0.37 QQ
NCS 13a 8.8 376 1.82 0.68 2.01 0.67 GQ
NCS 13b 2.9 120 2.48 0.25 2.00 0.21 QQ
Precipitation buffer in QQ changed to ethanol, leading to protocol Qq NCS 8a 13.9 596 1.88 0.73 1.74 0.79 GQ
NCS 8b 32.1 1379 1.25 0.93 1.28 0.98 Qq
NCS 9a 11.2 493 2.06 0.72 1.93 0.75 GQ
NCS 9b 27.4 1178 1.22 0.75 1.19 0.76 Qq
NCS 10a 12.0 502 2.07 0.88 2.01 0.92 GQ
NCS 10b 31.9 1371 1.47 0.99 1.44 1.01 Qq
NCS 4-1a 15.3 644 2.10 0.83 1.96 0.89 GQ
NCS 4-1b 26.1 1124 0.74 0.58 0.73 0.60 Qq
NCS 4-2a 24.7 1062 1.97 1.05 1.96 1.06 GQ
NCS 4-2b 49.9 2048 0.85 0.73 0.87 0.76 Qq
NCS 4-3a 28.5 1225 2.08 1.22 1.87 1.15 GQ
NCS 4-3b 46.9 1969 1.50 1.23 1.50 1.21 Qq
  1. Two punches, each having 9 mm diameter, were analyzed per DBS. Punches labeled “a” were tested with GQ while their matched pairs, labeled “b”, were tested with QQ or Qq. When the DNA precipitation buffer in QQ was changed to ethanol, the resultant protocol was termed Qq. Average eluate volume by GQ, QQ or Qq was 42 μl. Data represent averages of 2–4 readings per sample.