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Table 1 Combinations of Gensolve and Qiagen protocols for DNA extraction from DBS: GQ, QQ and Qq methods

From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling

      

65°C, 65 min

  
 

Sample code

DNA (ng/μl)

DNA (ng)

260/280

260/230

260/280

260/230

Protocol

 

NCS 11a

21.9

944

2.03

1.04

1.89

1.02

GQ

NCS 11b

3.4

147

1.55

0.19

1.35

0.29

QQ

NCS 12a

19.1

802

1.91

1.19

1.90

1.18

GQ

NCS 12b

6.1

257

1.58

0.36

1.73

0.37

QQ

NCS 13a

8.8

376

1.82

0.68

2.01

0.67

GQ

NCS 13b

2.9

120

2.48

0.25

2.00

0.21

QQ

Precipitation buffer in QQ changed to ethanol, leading to protocol Qq

NCS 8a

13.9

596

1.88

0.73

1.74

0.79

GQ

NCS 8b

32.1

1379

1.25

0.93

1.28

0.98

Qq

NCS 9a

11.2

493

2.06

0.72

1.93

0.75

GQ

NCS 9b

27.4

1178

1.22

0.75

1.19

0.76

Qq

NCS 10a

12.0

502

2.07

0.88

2.01

0.92

GQ

NCS 10b

31.9

1371

1.47

0.99

1.44

1.01

Qq

NCS 4-1a

15.3

644

2.10

0.83

1.96

0.89

GQ

NCS 4-1b

26.1

1124

0.74

0.58

0.73

0.60

Qq

NCS 4-2a

24.7

1062

1.97

1.05

1.96

1.06

GQ

NCS 4-2b

49.9

2048

0.85

0.73

0.87

0.76

Qq

NCS 4-3a

28.5

1225

2.08

1.22

1.87

1.15

GQ

NCS 4-3b

46.9

1969

1.50

1.23

1.50

1.21

Qq

  1. Two punches, each having 9 mm diameter, were analyzed per DBS. Punches labeled “a” were tested with GQ while their matched pairs, labeled “b”, were tested with QQ or Qq. When the DNA precipitation buffer in QQ was changed to ethanol, the resultant protocol was termed Qq. Average eluate volume by GQ, QQ or Qq was 42 μl. Data represent averages of 2–4 readings per sample.
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BMC Biotechnology

ISSN: 1472-6750

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