HM450 QC plot using Non-polymorphic probes which assess overall performance. In the green channel, background signals are shown in red and pink while positive signals in opaque and fluorescent green. In the red channel, background signals are shown in opaque and fluorescent green while positive signals in red and pink. One non-polymorphic control has been designed for each of the four nucleotides A, T, C, and G. Four DBS DNA samples are shown between four neonatal blood and four cell line DNA samples, in each of the two plots. The DBS samples represent two NCS spots, 37 and 38, each consisting of two tested punches labeled ‘a’ or ‘b’.