Figure 1From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profilingPhases and classification of protocols used to extract DNA from DBS. Two sequential phases, each encompassing three steps, are outlined (A) and were optimized in the different protocols or method combinations (B) used to extract DNA from DBS. A spin basket is shown next to Phase I.A and consists of a tube with an embedded perforated basket used to separate blood solutions from the filter papers from which they were extracted. A silica-gel column with a funnel-shape design is shown next to Phase II.E and often used to elute small volumes (5–30 μl), as manufactured by Macherey-Nagel and supplied with the extra-small (XS) versions of NucleoSpin kits (B).Back to article page