High-throughput strategies for penicillin G acylase production in rE. colifed-batch cultivations

BMC Biotechnology

Table 4 PGA production in various E. coli expression systems for bioreactor cultures carried out under different induction strategies

*E. coli*strain/construction	Ind.	Op. mode	T_ind(°C)	[Biomass] (gDCW L^-1)	EA_sp(IU DCW^-1)	Vol. Enz.Act. (IU mL^-1)	Product. (IU mL^-1 h^-1)	Ref.
RE3/ pKA18	Phenyl acetic acid	Batch	28	5	1000	4.5	0.28*	[33]
MDDP7/ pTrcKnPAC2902	IPTG	Fed-batch	28	33	1020*	37.5	0.48*	[6]
W3110/pPA102	Cheese whey	Batch	29	1.5	781	0.901	0.053*	[23]
9633/pGL-5	IPTG	Fed-batch	30	96.9*	157*	15	0.63*	[24]
BL21(DE3)/pET28b	IPTG	Batch	28	12	800	10	0.42*	[21]
DH5α/ Psmlfpga	IPTG	Batch	30	21.7*	153 mg gDCW^-1	79.88	0.67*	[22]
DH5α/pET30b	Constitutive	Batch	28.5	4.5	NA	43	2.58*	[20]
BL21(DE3)/pT101/D-TOPO	Lactose	Continuous fed-batch	20	100	2000	190	7.8	This work
BL21(DE3)/pT101/D-TOPO	Lactose	Intermittent fed-batch	20	38	2800	94	3.9	This work

ISSN: 1472-6750