Table 2 Results obtained for the batch adsorption-desorption process of PGA from recombinant E. coli using the Streamline SP XL resin at pH 4.8
From: High-throughput strategies for penicillin G acylase production in rE. colifed-batch cultivations
Samples | Cells harvested from complex medium | Cells harvested from defined medium | ||||
|---|---|---|---|---|---|---|
Enz. Act. (IU L-1) | Prot. Conc. (mgprotL-1) | Spec. Act. (IU mgprot-1) | Enz. Act. (IU L-1) | Prot. Conc. (mgprotL-1) | Spec. Act. (IU mgprot-1) | |
Clarified | 500 ± 45 | 480.8 ± 32.1 | 1.04 ± 0.70 | 420 ± 51 | 305.1 ± 15.6 | 1.38 ± 0.42 |
Extract | 130 ± 21 | 213.1 ± 43.4 | 0.61 ± 0.07 | 150 ± 32 | 235.1 ± 43.1 | 0.64 ± 0.21 |
Washing | 0.2 ± 0.1 | 3 ± 1 | 0.3 ± 0.2 | 0.2 ± 0.1 | 0.4 ± 0.1 | 0.1 ± 0.3 |
Eluate | 1200 ± 82 | 510.6 ± 14.2 | 2.35 ± 0.03 | 893 ± 76 | 231.1 ± 16.2 | 3.86 ± 0.34 |
ηactel (%) | 97 ± 2 | 99 ± 2 | ||||
ηactads (%) | 74 ± 6 | 64 ± 4 | ||||
ηprocess (%) | 72 ± 5 | 64 ± 6 | ||||
P.F. | 2.3 ± 0.3 | 2.8 ± 0.4 | ||||