Table 1 Composition of media used in bioreactor cultivations
From: High-throughput strategies for penicillin G acylase production in rE. colifed-batch cultivations
Auto-induction | Complex media | Defined media | ||||
|---|---|---|---|---|---|---|
Nutrient (g L-1) | Batch | Pulse | Batch | Fed-batch | Batch | Fed-batch |
Glucose | 10.0 | --- | 10.0 | --- | --- | --- |
Glycerol | 60.0 | 258.3 | 40.0 | 800 | 40.0 | 800.0 |
Lactose | 20 | 130.0 | --- | --- | --- | --- |
Tryptone | 10.0 | 10.0 | 10.0 | 10.0 | --- | --- |
Yeast extract | 5.0 | 5.0 | 5.0 | 5.0 | --- | --- |
MgSO4.7H2O | 0.5 | 0.5 | 0.5 | 40.0 | 1.6 | 20.0 |
KH2PO4 | 3.4 | 3.4 | 3.4 | 3.4 | 17.7 | 21.28 |
(NH4)2HPO4 | --- | --- | --- | --- | 5.3 | 6.4 |
Na2HPO4.12H2O | 9.0 | 9.0 | 9.0 | 9.0 | --- | --- |
Citric acid | --- | --- | --- | --- | 2.27 | 2.27 |
NH4Cl | 2.7 | 2.7 | 2.7 | 2.7 | --- | --- |
Na2SO4 | 0.7 | 0.7 | 0.7 | 0.7 | --- | --- |
Thiamine | --- | --- | --- | --- | 45.0 | 45.0 |