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Figure 1 | BMC Biotechnology

Figure 1

From: High-throughput strategies for penicillin G acylase production in rE. colifed-batch cultivations

Figure 1

Effect of induction temperature upon specific activity (Eq. 4 ) and volumetric enzyme activity (Eq. 5 ) of PGA produced by recombinant E. coli grown in shake flasks containing LB medium after 24 h of induction. All experiments induced with 0.25 mM IPTG, Tgrowth = 37°C, ODinduction = 1.5. Error bars for specific enzymatic activity, volumetric enzyme activity and biomass concentration are standard deviations from triplicates. Means with different letters (a–e) differ significantly according to the Tukey’s test (p <0.05).

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