Figure 3From: Production and characterization of in planta transiently produced polygalacturanase from Aspergillus nigerand its fusions with hydrophobin or ELP tagsDeglycosylation analysis of plant-expressed An PGI targeted to different compartments. Purified AnPGI was incubated for 3Â h in denaturing conditions in the absence (-) or presence (+) of EndoH, analysed by SDS-PAGE under reducing conditions, and subjected to Western blot analysis with anti-C-myc antibody.Back to article page