Accumulation and activity of An PGI in different subcellular compartiments. A) Accumulation of AnPGI in different subcellular compartments. B) A N. benthamiana agroinfiltrated leaf disc was incubated in 50 mM sodium acetate at 50°C for 24 h and used to analyze self hydrolysis and release of reducing sugars using the dinitro salicylic acid (DNS) method. C) Purified AnPGI was used to confirm the difference in polygalacturonase activities when targeted to different subcellular compartments using polygalacturonic acid as the substrate. Accumulation results represent the average of AnPGI levels in five different plants, and the release of reducing sugars and protein activity were determined in triplicate. Reducing sugar concentration was normalized using the assay results for wild-type plants. Error bars represent ± standard error.