Figure 1From: Production and characterization of in planta transiently produced polygalacturanase from Aspergillus nigerand its fusions with hydrophobin or ELP tagsSchematic representation of the diverse An PGI heterologous expression constructs evaluated in this study: A) constructs used for protein targeting experiments and B) constructs used to analyze the effect of fusion tags on protein expression. p35S, double enhanced 35S promoter from Cauliflower Mosaic Virus 35S gene; tCUP, translation enhancer from the tobacco cryptic upstream promoter; nos, nopaline synthase transcription terminator; Pr1b, tobacco pathogenesis related 1b protein secretory signal peptide; C-myc, detection/purification tag; KDEL, endoplasmic reticulum retrieval tetrapeptide; CTPP, vacuole sorting peptide; RuBisCo T.P., rubisco small subunit transit peptide; HFBI, hydrophobin I; ELP, elastin-like polypeptide. Schematic not drawn to scale.Back to article page