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Figure 1 | BMC Biotechnology

Figure 1

From: Improved elongation factor-1 alpha-based vectors for stable high-level expression of heterologous proteins in Chinese hamster ovary cells

Figure 1

Map of the p1.1 plasmid vector and the cloning scheme for p1.1-based plasmids. A. Plasmid map. UFR: upstream flanking region of the EEF1A gene; DFR: downstream flanking region; PL: polylinker region; pA: polyadenylation signal; bla – ampicillin resistance gene; bla prom – promoter of the ampicillin resistance gene. B. Cloning scheme for p1.1-based plasmids. Generation of cloning inserts by PCR with adaptor primers is depicted by dashed lines; generation of cloning inserts by restriction is depicted by solid lines. EBV F1-6: corresponding synthetic fragments of the EBVTR element. 5CH F1-6: corresponding fragments of the upstream flanking region of the EEF1A gene; 3CH F1-6: corresponding fragments of the downstream flanking region of the EEF1A gene.

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