Figure 2
Dystrophin immunostaining (red) showing the engraftment of transplanted myoblasts in dystrophin-deficient mice. A TA muscle was injected with 5 × 105 cells and harvested 2 months after transplantation. (A) TA muscle of mdx mouse injected with GFP-labeled myoblasts. (B) TA muscle of GFP transgenic-mdx hybrid mouse injected with non-labeled myoblasts. (C) Merged image of A with GFP. (D) Merged image of B with GFP. Scale bar = 20 μm. (E) Levels of dystrophin-positive fibers in TA muscle of mdx mouse injected with GFP-labeled myoblasts (bar A) and of GFP transgenic-mdx hybrid mouse injected with non-labeled myoblasts (bar B). Values are mean ± standard errors of 5 samples. (*P < 0.01; Student’s t-test).