Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coliand the enzyme characteristics

Table 3 The effect of metal ions and reagents on the activity of rADI

Metal ions/Reagents	Relative remaining activity (%) of rADI
	1 mM	10 mM
Control	100.0 ± 2.9	100.0 ± 2.4
Li⁺	99.6 ± 2.5	100.5 ± 0.8
Na⁺	100.1 ± 3.5	102.5 ± 4.4
K⁺	104.1 ± 2.5	100.1 ± 4.9
Mg²⁺	96.4 ± 3.2	102.1 ± 1.5
Ca²⁺	96.2 ± 3.9	91.2 ± 2.4
Mn²⁺	97.4 ± 2.1	101.5 ± 4.2
Cr³⁺	98.1 ± 2.3	81.5 ± 1.9
Co²⁺	94.9 ± 2.8	73.7 ± 2.5
Ni²⁺	100.6 ± 1.9	90.1 ± 0.1
Cu²⁺	80.2 ± 1.2	33.6 ± 1.5
EDTA	100.3 ± 4.6	94.9 ± 1.8
SDS	22.2 ± 2.5	4.9 ± 1.6
DTT	71.0 ± 0.3	13.6 ± 1.2

ISSN: 1472-6750