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Table 2 Effects of combined additions of different sugars and amino acids on the enzymatic activities of rADI condition optimization

From: Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coliand the enzyme characteristics

Combined additiona

Enzymatic activityb(U/ml)

Amino acid

Percentage

Sugar

Percentage

 
   

0%

2.08 ± 0.05

   

0.5%

9.43 ± 0.02

L-arginine 0.5%

D-glucose

1%

1.76 ± 0.01

   

2%

1.60 ± 0.06

   

5%

1.41 ± 0.01

 

0%

  

2.78 ± 0.02

 

0.5%

  

9.33 ± 0.04

L-arginine

1%

D-glucose 0.5%

0.86 ± 0.04

 

2%

  

0.74 ± 0.03

 

5%

  

0.70 ± 0.05

 

0%

  

2.92 ± 0.03

 

0.5%

  

2.93 ± 0.05

L-arginine hydrochloride

1%

D-glucose 0.5%

3.85 ± 0.07

 

2%

  

4.35 ± 0.02

 

5%

  

3.95 ± 0.04

 

0%

  

3.18 ± 0.03

 

0.5%

  

3.20 ± 0.08

L-arginine hydrochloride

1%

-

3.31 ± 0.05

 

2%

  

3.60 ± 0.02

 

5%

  

3.20 ± 0.08

  

-

-

3.26 ± 0.07

  

D-glucose

0.5%

9.31 ± 0.01

L-arginine 0.5%

D-galactose

0.5%

2.35 ± 0.04

  

D-maltose

0.5%

1.96 ± 0.04

  

D-sucrose

0.5%

2.49 ± 0.10

-

-

  

3.22 ± 0.06

L-arginine

0.5%

  

9.39 ± 0.03

L-tryptophan

0.5%

D-glucose 0.5%

1.93 ± 0.06

L-citrulline

0.5%

  

1.97 ± 0.01

L-glycine

0.5%

  

2.69 ± 0.02

  1. a: with the presence of pGro7; b: produced from crude extracts.