Table 2 Conjugation frequencies per 10 8 spores of integrating plasmids into Streptomyces species
From: A novel Streptomyces spp. integration vector derived from the S. venezuelaephage, SV1
Streptomycesrecipient: | E. colidonor, ET12567 (pUZ8002) containing plasmids: | |||
|---|---|---|---|---|
|  | pBF3 (SV1 g27/attP) | pMS82 (ϕBT1 int/attP) | pSET152 (φC31 int/attP) | pRT801 (φBT1 int/attP) |
S. coelicolor J1929 | 2.5 × 106 | 3.6 × 106 | 6 × 105 | ND1 |
S. coelicolor M512 | 1.7 × 107 | 6 × 107 | 6 × 106 | ND |
S. lividans | 1.4 × 105 | 9.5 × 105 | 2.3 × 105 | ND |
S. venezuelae 10712 | 3.3 × 104 | 1.7 × 107 | 9.8 x 104 | ND |
S. avermitilis | 40 | 3 × 105 | 1.8 x 103 | ND |
S. albus J1074 | 4.6 × 102 | 5 × 105 | 1 x 103 | ND |
S. coelicolor M512: pBF3 | - | - | 4 x 106 | 2.8 x 105 |