Table 1 Conjugation frequnecies of various integrating plasmids into S. coelicolor J1929
From: A novel Streptomyces spp. integration vector derived from the S. venezuelaephage, SV1
E. coliET12567(pUZ8002) donor containing: | Origin of integrase and attP | Hygromycin resistant exconjugants/108spores |
|---|---|---|
pMS98 | SV1 | 2.4 × 106 |
pBF1 | SV1 | 1.1 × 107 |
pBF1* | SV1 | 9.9 × 106 |
pBF3 | SV1 | 1.4 × 107 |
pEY25 | φC31 (int only) | 9 x103 |