Skip to main content

Table 5 Marker-assisted transformations of single R genes to different varieties

From: Development of late blight resistant potatoes by cisgene stacking

Inserted Rgene Variety Plant ID Explants # Regeneration time (days) shoots % shoots # rooting % PCR + % frequency % vbf % vbf in DLA # vbf and R in DLA #
Rpi-vnt1.1 Atlantic H13 200 60-120 76 30a 100 100 76 40 12 12
Rpi-sto1   H09 200 60-120 66 30a 100 100 66 50 15 15
Rpi-vnt1.1 Bintje F13 200 60-120 13 26b 77 100 10 40 8 8
Rpi-sto1   F09 200 60-120 10 20b 100 100 10 45 9 9
Rpi-vnt1.1 Potae9 W13 200 60-120 16 31b 84 100 13 47 12 12
Rpi-sto1   W09 200 60-120 19 37b 61 100 11 39 9 9
  1. #exp; number of explants, % sht; percentage of number of shoots over number of explants, % rt; percentage of number of rooted shoots over the number of shoots, % PCR+; percentage of PCR positive shoots over the number of shoots, % freq; transformation frequency, calculated by % sht × % rt × % PCR+, a Among regenerated shoots, 30 plants were tested. b All regenerated shoots were tested. % vbf; percentage of backbone free plants out of plants tested. #vbf plants DLA: number of vector backbone free plants tested in detached leaf assays. #vbf R plants DLA: number of vector backbone free resistant plants in detached leaf assay.