Development of late blight resistant potatoes by cisgene stacking

BMC Biotechnology

Table 5 Marker-assisted transformations of single R genes to different varieties

Inserted Rgene	Variety	Plant ID	Explants #	Regeneration time (days)	shoots %	shoots #	rooting %	PCR + %	frequency %	vbf %	vbf in DLA #	vbf and R in DLA #
Rpi-vnt1.1	Atlantic	H13	200	60-120	76	30^a	100	100	76	40	12	12
Rpi-sto1		H09	200	60-120	66	30^a	100	100	66	50	15	15
Rpi-vnt1.1	Bintje	F13	200	60-120	13	26^b	77	100	10	40	8	8
Rpi-sto1		F09	200	60-120	10	20^b	100	100	10	45	9	9
Rpi-vnt1.1	Potae9	W13	200	60-120	16	31^b	84	100	13	47	12	12
Rpi-sto1		W09	200	60-120	19	37^b	61	100	11	39	9	9

#exp; number of explants, % sht; percentage of number of shoots over number of explants, % rt; percentage of number of rooted shoots over the number of shoots, % PCR+; percentage of PCR positive shoots over the number of shoots, % freq; transformation frequency, calculated by % sht × % rt × % PCR+, a Among regenerated shoots, 30 plants were tested. b All regenerated shoots were tested. % vbf; percentage of backbone free plants out of plants tested. #vbf plants DLA: number of vector backbone free plants tested in detached leaf assays. #vbf R plants DLA: number of vector backbone free resistant plants in detached leaf assay.

ISSN: 1472-6750