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Table 4 Phenotypic characterization of vector backbone free (cisgenic) events in different potato varieties carrying the Rpi-vnt1 and Rpi-sto1 genes

From: Development of late blight resistant potatoes by cisgene stacking

Cisgenic event

Variety

Plant morphology

Agroinfiltration

DLA

Avrvnt1

Avrsto1

EC1

IPO-C

DHD11

90128

PIC99189

H43-1

Atlantic

 

+

–

S

R

R

R

R

H43-2

Atlantic

curly leaf

n

n

n

n

n

n

n

H43-3

Atlantic

curly leaf

n

n

n

n

n

n

n

H43-4

Atlantic

 

–

–

S

S

S

S

S

H43-7

Atlantic

 

+

+

R

R

R

R

R

H43-8

Atlantic

 

+

+

R

R

R

R

R

H43-10

Atlantic

 

+

–

S

R

R

R

R

H43-11

Atlantic

curly leaf

n

n

n

n

n

n

n

H43-12

Atlantic

 

+

–

S

S

S

S

S

F43-1

Bintje

 

+

–

S

R

R

R

R

F43-2

Bintje

 

+

+

R

R

R

R

R

F43-3

Bintje

 

+

+

R

R

R

R

R

F43-4

Bintje

 

+

+

R

R

R

R

R

F43-5

Bintje

 

+

+

R

R

R

R

R

W43-1

Potae9

 

+

+

R

R

R

R

R

W43-2

Potae9

curly leaf

n

n

n

n

n

n

n

W43-3

Potae9

 

+

–

S

R

R

R

R

W43-4

Potae9

dwarf

n

n

n

n

n

n

n

W43-5

Potae9

 

+

+

R

R

R

R

R

  1. Cisgenic events functionally expressing both R genes were highlighted by bold font. n: no data, +: responsive to infiltration with the indicated Avr gene; -: not responsive to infiltration with the indicated Avr gene R: resistant to the indicated isolate in detached leaf assays (DLA). S: susceptible to the indicated isolate in DLA.