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Table 3 Marker-free transformation of two R genes ( Rpi-vnt1.1:Rpi-sto1 ) to different potato varieties; Identification of PCR-positive shoots in different time ranges after marker-free transformation

From: Development of late blight resistant potatoes by cisgene stacking

Variety

31-50 days

51-70 days

71-90 days

91-110 days

111-130 days

Total

Atlantic

4/197

4/174

4/111

0/15

0/0

12/497

Bintje

2/199

3/194

1/165

0/32

0/0

6/590

Potae9

4/183

2/143

1/78

0/24

0/0

7/428

  1. Number of PCR-positive shoots carrying both Rpi-sto1 and Rpi-vnt1.1 over the number of tested shoots.