Functional validation of cisgenic transformants by agroinfiltration and resistance assays. A. Avrvnt1- and Avrsto1-induced hypersensitive responses in cisgenic transformant H43-7 (Rpi-vnt1:Rpi-sto1 in Atlantic background). Avrvnt1 and Avrsto1 were infiltrated in cisgenic plants. A 1:1 mixture of R3a and Avr3a and pK7WG2 were infiltrated as positive and negative controls, respectively. B. Detached leaf assays for cisgenic transformant H43-7. Different isolates are shown in the middle. Cisgenic transformant are shown on the top and the wild type Atlantic on the bottom of the panel.