Schematic diagram of the marker-free double gene construct pBINAW2: Rpi-vnt1.1:Rpi-sto1 . In light green and light blue arrows the Rpi-vnt1.1 and Rpi-sto1 genes are shown, respectively. The red arrows indicate the coding regions of Rpi-vnt1.1 or Rpi-sto1. Unique restriction enzyme recognition sites XmaI, SbfI and AscI are shown. RB: right border of T-DNA, LB: left border of T-DNA, TetA, trfA, NPTIII, ColE1, oriV and traJ are vector backbone sequences for plasmid stability and replication in bacterial hosts Agrobacterium tumefaciens and Escherichia coli.